Sperm metabolism of the teleost fishes chalcalburnus
The analysis of sperm motility has enhanced our knowledge of both sperm biology and the assessment of semen quality. The development of computer-assisted sperm analysis CASA has improved the utility of such studies, allowing tracking individual spermatozoa and obtaining objective and repeatable data. However, only recently have multivariate statistical analyses been used, taking advantage of the large quantity of data generated by CASA. These analyses allow classifying heterogeneous semen samples into homogeneous subpopulations, grouping spermatozoa with similar motility patterns.
We explore the possibility of characterizing sperm cells without the need to stain them using spectral and fluorescence lifetime analyses after multi-photon excitation in an insect model. The autofluorescence emission spectrum of sperm of the common bedbug, Cimex lectularius , was consistent with the presence of flavins and NAD P H. The fluorescence lifetime histograms revealed four peaks. These peaks 0.
E-mail address: Franc. Lahnsteiner sbg. Use the link below to share a full-text version of this article with your friends and colleagues. Learn more.
To browse Academia. Skip to main content. You're using an out-of-date version of Internet Explorer. Log In Sign Up. Metabolism of intratesticular spermatozoa of a tropical teleost fish Clarias gariepinus Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology,